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Source: University of Otago

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Genetic information is a critical part of conservation research but has proven invasive and costly – until now.
A University of Otago study compared traditional tissue sampling with environmental DNA sampling to obtain population genetic information. It showed there is potential for this new approach to help with conservation management.
eDNA is an effective tool to monitor the presence of species in water – as an animal swims by in the ocean, it releases DNA into the environment through excrement, mucus, or the shedding of scales which can be collected and analysed by researchers.
Dr Clare Adams
Study lead author Dr Clare Adams, of the Coastal People: Southern Skies Centre of Research Excellence, says the group wanted to push this method one step further, obtaining genetic information on a population, specifically blackfoot pāua.
“We wanted to determine how well eDNA methodologies could be used to monitor populations of marine species.
“Conservation research has shown that genetic information is useful to support management decisions, but it is difficult to come by and involves invasive sampling of the very species you are hoping to protect and manage.
“eDNA methods are increasingly cheaper, easier, and less time consuming than traditional genetic sampling, so they could be used to help monitor how genetically healthy and biodiverse populations of target organisms may be,” she says.
The study, published in Environmental DNA, took tissue samples of pāua and water samples from near the pāua colonies.
Dr Adams says eDNA proved to be an effective method in analysing common genetic variations, though rare genetic variants (present in less than 5 per cent of the population) were more difficult to detect.
The researchers focused on pāua due to its cultural importance to Māori and commercial importance to the global fisheries industry.
“Genetic diversity is essential to monitor because it can tell us about how inbred pāua stocks might be, as well as hinting at gene flow, and ultimately can highlight mechanisms of evolution.
“We hope some of the work done here can help communities monitor their pāua stock.”
Publication details:
Environmental DNA reflects common haplotypic variation
Clare I. M. Adams, Christopher Hepburn, Gert-Jan Jeunen, Hugh Cross, Helen R. Taylor, Neil J. Gemmell, Michael Bunce, Michael Knapp
Environmental DNA
For more information, please contact:
Dr Clare AdamsCoastal People, Southern SkiesDepartments of Anatomy, Zoology, and Marine ScienceEmail
Ellie RowleyCommunications AdviserUniversity of OtagoMob +64 21 278 8200Email